mar560 - Profile Module Fermentation (Complete module description)
| Module label | Profile Module Fermentation |
| Module code | mar560 |
| Credit points | 6.0 KP |
| Workload | 180 h |
| Institute directory | Institute for Chemistry and Biology of the Marine Environment |
| Applicability of the module |
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| Responsible persons |
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| Prerequisites | |
| Skills to be acquired in this module | The students are getting directly involved in actual scientific projects in the area of general physiology (under guidance). They understand the scientific rational and design of the experiment(s), get acquainted with state-of-the-art concepts and technologies for growth balancing (e.g. bioreactor), know how to write concise scientific protocols, know how to present/discuss their results in public. |
| Module contents | "Growth balancing": Daily lectures introduce the students to theory and concepts of growth stoichiometry: (i) aerobic or anaerobic growth experiments in glass vessels and/or bioreactors, (ii) experimental design, (iii) design and operating laboratory fermenters, (iv) HPLC, IC and GC-MS analysis. Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project. The following sequence of experiments will be conducted: - cultivation of bacterial pure cultures in Erlenmeyer flasks, glass bottles or controlled bioreactors - determination of optical density, the live count, dry weight of cells and microscopic inspection during cultivation - (dis)assembly and sterilization of fermentation devices - operate process-controlled fermenters (incl. O2 and pH adjustments and sterile sampling) - determine O2-consumption and CO2-production rates based on on-line GC-MS measurements - quantification of substrate consumption for HPLC and IC - quantitative determination and calculation growth balances and efficiencies |
| Recommended reading | Will be announced in the courses. |
| Links | |
| Language of instruction | English |
| Duration (semesters) | 1 Semester |
| Module frequency | not offered at the moment |
| Module capacity | unlimited |
| Type of module | Wahlpflicht / Elective |
| Module level | AC (Aufbaucurriculum / Composition) |
| Teaching/Learning method | Seminar (1 SPPW); practical course (4 SPPW) |
| Previous knowledge | Lecture: Physiology and diversity of prokaryotes (successfully completed); Lecture: Molecular Microbiology |
| Type of course | Comment | SWS | Frequency | Workload of compulsory attendance |
|---|---|---|---|---|
| Seminar | 1 | 14 | ||
| Practical training | 4 | 56 | ||
| Total module attendance time | 70 h | |||
| Examination | Prüfungszeiten | Type of examination |
|---|---|---|
| Final exam of module | Announced at the beginning of the course. |
One assessment of examination: |
