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University of Oldenburg
04.12.2022 15:48:26
mar560 - Profile Module Fermentation (Complete module description)
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Module label Profile Module Fermentation
Modulkürzel mar560
Credit points 6.0 KP
Workload 180 h
Institute directory Institute for Chemistry and Biology of the Marine Environment
Verwendbarkeit des Moduls
  • Master's Programme Microbiology (Master) > Mastermodule
Zuständige Personen
Rabus, Ralf Andreas (Module responsibility)
Wöhlbrand, Lars (Module counselling)
Prerequisites
Lecture: Physiology and diversity of prokaryotes (successfully completed) Lecture: Molecular Microbiology
Skills to be acquired in this module
The students are getting directly involved in actual scientific projects in the area of general physiology (under guidance). They understand the scientific rational and design of the experiment(s), get acquainted with state-of-the-art concepts and technologies for growth balancing (e.g. bioreactor), know how to write concise scientific protocols, know how to present/discuss their results in public.
Module contents
"Growth balancing": Daily lectures introduce the students to theory and concepts of growth stoichiometry: (i) aerobic or anaerobic growth experiments in glass vessels and/or bioreactors, (ii) experimental design, (iii) design and operating laboratory fermenters, (iv) HPLC, IC and GC-MS analysis. Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project. The following sequence of experiments will be conducted: - cultivation of bacterial pure cultures in Erlenmeyer flasks, glass bottles or controlled bioreactors - determination of optical density, the live count, dry weight of cells and microscopic inspection during cultivation - (dis)assembly and sterilization of fermentation devices - operate process-controlled fermenters (incl. O2 and pH adjustments and sterile sampling) - determine O2-consumption and CO2-production rates based on on-line GC-MS measurements - quantification of substrate consumption for HPLC and IC - quantitative determination and calculation growth balances and efficiencies
Literaturempfehlungen
Links
Language of instruction English
Duration (semesters) 1 Semester
Module frequency jährlich
Module capacity unlimited
Modullevel / module level
Modulart / typ of module Wahlpflicht / Elective
Lehr-/Lernform / Teaching/Learning method Seminar (1 SPPW); practical course (4 SPPW)
Vorkenntnisse / Previous knowledge Lecture: Physiology and diversity of prokaryotes (successfully completed); Lecture: Molecular Microbiology
Form of instruction Comment SWS Frequency Workload of compulsory attendance
Seminar 1 14
Practical training 4 56
Präsenzzeit Modul insgesamt 70 h
Examination Prüfungszeiten Type of examination
Final exam of module
Announced at the beginning of the course.

One assessment of examination: Portfolio (seminar presentation, written protocol) Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)