Stud.IP Uni Oldenburg
Universität Oldenburg
29.11.2021 09:29:18
mar610 - Profile Module Isolation and characterization of microorganisms (Vollständige Modulbeschreibung)
Originalfassung Englisch PDF Download
Modulbezeichnung Profile Module Isolation and characterization of microorganisms
Modulkürzel mar610
Kreditpunkte 6.0 KP
Workload 180 h
Einrichtungsverzeichnis Institut für Chemie und Biologie des Meeres (ICBM)
Verwendbarkeit des Moduls
  • Master Microbiology (Master) > Mastermodule
Zuständige Personen
Könneke, Martin (Modulverantwortung)
Engelen, Bert (Modulberatung)
In this course the students will isolate bacteria and other microorganisms. They will learn classical microbiological techniques as enrichment culture, aseptic work, preparation of liquid and solid media, cultivation under oxic and anoxic condition, on agar plates and in deep agar dilution, description of microbes by techniques as staining, microscopy, microphotography.

Prior to the laboratory work the participants shall read literature about first isolation, description and current studies on their target organisms and present this and their isolation strategy in the seminar. During the course and at the end, results and a possible molecular identification of isolates will be presented and discussed.

Practical work: Every student prepares media and agar plates required for the isolation of the different target organisms. If pure cultures have been isolated, they should be transferred to long-term storage on agar and in liquid nitrogen. Sampling sites and different stages of the enrichment and isolation are documented by macro- and microphotography and described in the report. Finally, tests to verify purity of the culture and its identification, as well as a phylogenetic analysis are requested.
Brock “Biology of Microorganisms”, Cypionka “Grundlagen der Mikrobiologie”, Drews “Mikrobiologisches Praktikum”, DSMZ catalogue (, Dyer “A field guide to the bacteria”, Reddy “Methods for general and molecular Microbiology”, Steinbüchel “Mikrobiologisches Praktikum”,
Unterrichtssprache Englisch
Dauer in Semestern 1 Semester
Angebotsrhythmus Modul jährlich
Aufnahmekapazität Modul unbegrenzt
Modullevel / module level MM (Mastermodul / Master module)
Modulart / typ of module Wahlpflicht / Elective
Lehr-/Lernform / Teaching/Learning method Seminar and laboratory work, twice per week, half a day each
Vorkenntnisse / Previous knowledge Module mar500 including lectures on “Physiology and life modes of prokaryotes” and “Microbial diversity”
Lehrveranstaltungsform Kommentar SWS Angebotsrhythmus Workload Präsenz
1 14
4 56
Präsenzzeit Modul insgesamt 70 h
Prüfung Prüfungszeiten Prüfungsform
Will be announced during the course

One assessment of examination: Portfolio (seminar presentation, written protocol)

Protocol (100 %), webpage, seminar presentation (no mark).

Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)