mar610 - Profile Module Isolation and characterization of microorganisms (Complete module description)

Module label	Profile Module Isolation and characterization of microorganisms
Modulkürzel	mar610
Credit points	6.0 KP
Workload	180 h
Institute directory	Institute for Chemistry and Biology of the Marine Environment
Verwendbarkeit des Moduls	Master's Programme Microbiology (Master) > Mastermodule
Zuständige Personen	Könneke, Martin (module responsibility) Engelen, Bert (Module counselling)
Prerequisites
Skills to be acquired in this module	In this course the students will isolate bacteria and other microorganisms. They will learn classical microbiological techniques as enrichment culture, aseptic work, preparation of liquid and solid media, cultivation under oxic and anoxic condition, on agar plates and in deep agar dilution, description of microbes by techniques as staining, microscopy, microphotography.
Module contents	Prior to the laboratory work the participants shall read literature and current studies about their target group of microorganisms and develop an enrichment strategy isolation. They will present this and their enrichment strategy in the seminar. During the course and at the end, results and a possible molecular identification of isolates will be presented and discussed. Practical work: Student prepares media and agar plates required for the enrichment and isolation of the different target organisms in small groups. The enrichment cultures will be monitored over time by measuring various biological and chemical parameters. If pure cultures have been isolated, they will be analysed microscopically and identified using molecular methods.
Literaturempfehlungen	Brock “Biology of Microorganisms” Cypionka “Grundlagen der Mikrobiologie” Drews “Mikrobiologisches Praktikum” DSMZ catalogue (www.dsmz.de) yer “A field guide to the bacteria” Reddy “Methods for general and molecular Microbiology” Steinbüchel “Mikrobiologisches Praktikum” www.microbiological-garden.net
Links	www.microbiological-garden.net
Language of instruction	English
Duration (semesters)	1 Semester
Module frequency	annual
Module capacity	unlimited
Type of module	Wahlpflicht / Elective
Module level	AC (Aufbaucurriculum / Composition)
Teaching/Learning method	Seminar and laboratory work, twice per week, half a day each
Previous knowledge	Module mar500 including lectures on “Physiology and life modes of prokaryotes” and “Microbial diversity”

Lehrveranstaltungsform	SWS	Frequency	Workload of compulsory attendance
Seminar	1	SoSe	14
Exercises	4	SoSe	56
Präsenzzeit Modul insgesamt			70 h

Examination	Prüfungszeiten	Type of examination
Final exam of module	Announced during the course.	One assessment of examination: Portfolio: Protocol (100 %), seminar presentation (no mark) Active participation: Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.