Stud.IP Uni Oldenburg
University of Oldenburg
28.11.2023 20:05:36
mar520 - Main Module Proteomics (Complete module description)
Original version English PDF download
Module label Main Module Proteomics
Module abbreviation mar520
Credit points 12.0 KP
Workload 360 h
Institute directory Institute for Chemistry and Biology of the Marine Environment
Applicability of the module
  • Master's Programme Microbiology (Master) > Mastermodule
Responsible persons
  • Rabus, Ralf Andreas (module responsibility)
  • Feenders, Christoph (Module counselling)
  • W√∂hlbrand, Lars (Module counselling)
Prerequisites
Lecture: Physiology and diversity of prokaryotes
Lecture: Molecular Microbiology
Skills to be acquired in this module
The students are getting directly involved in actual scientific projects in the area of physiological and/or meta-proteomics (under guidance). They - get acquainted with state-of-the-art proteomic concepts and technologies, - know how to write concise scientific protocols, - know how to present/discuss their results in public.
Module contents
Daily lectures introduce the students to theory and concepts of modern proteomics: (i) separation of cellular compartments and protein extraction, (ii) gel-based and -free protein separation, (iii) gel-staining, protein detection and quantification by image analysis, (iv) integrative mass spectrometry-based protein identification, (v) meta-proteomics, and (vi) focused genomic analysis. Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project. The following sequence of experiments will be conducted: - extraction and quantification of total protein from prepared cell samples (incl. separation of compartments), - protein separation by SDS-PAGE and staining with Coomassie, silver and/or fluorescent dyes, - digital image acquisition and analysis, - manual and/or automated band excision, - protein identification by nanoLC-ESI-MS/MS, - nanoLC-MALDI-coupling and protein identification by MALDI-TOF-MS/MS, - Physiological interpretation of predicted protein functions and relevant genomic context.
Recommended reading
Lottspeich - Bioanalytik
Links
Language of instruction English
Duration (semesters) 1 Semester
Module frequency yearly
Module capacity unlimited
Reference text
Module level
Type of module je nach Studiengang Pflicht oder Wahlpflicht
Teaching/Learning method Seminar (2 CP ), practical course (10 CP)
Previous knowledge
Type of course Comment SWS Frequency Workload of compulsory attendance
Seminar 2 28
Practical training 8 112
Total module attendance time 140 h
Examination Examination times Type of examination
Final exam of module
Announced at the beginning of the course.

One assessments of examination: Portfolio: Written protocol and contribution to the seminar (seminar presentation) Seminar presentation (25%), written protocol (75 %). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice or the course.