mar520 - Main Module Proteomics (Complete module description)
| Module label | Main Module Proteomics |
| Module abbreviation | mar520 |
| Credit points | 12.0 KP |
| Workload | 360 h |
| Institute directory | Institute for Chemistry and Biology of the Marine Environment |
| Applicability of the module |
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| Responsible persons |
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| Prerequisites | Lecture: Physiology and diversity of prokaryotes Lecture: Molecular Microbiology |
| Skills to be acquired in this module | The students are getting directly involved in actual scientific projects in the area of physiological and/or meta-proteomics (under guidance). They - get acquainted with state-of-the-art proteomic concepts and technologies, - know how to write concise scientific protocols, - know how to present/discuss their results in public. |
| Module contents | Daily lectures introduce the students to theory and concepts of modern proteomics: (i) separation of cellular compartments and protein extraction, (ii) gel-based and -free protein separation, (iii) gel-staining, protein detection and quantification by image analysis, (iv) integrative mass spectrometry-based protein identification, (v) meta-proteomics, and (vi) focused genomic analysis. Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project. The following sequence of experiments will be conducted: - extraction and quantification of total protein from prepared cell samples (incl. separation of compartments), - protein separation by SDS-PAGE and staining with Coomassie, silver and/or fluorescent dyes, - digital image acquisition and analysis, - manual and/or automated band excision, - protein identification by nanoLC-ESI-MS/MS, - nanoLC-MALDI-coupling and protein identification by MALDI-TOF-MS/MS, - Physiological interpretation of predicted protein functions and relevant genomic context. |
| Recommended reading | Lottspeich - Bioanalytik |
| Links | |
| Language of instruction | English |
| Duration (semesters) | 1 Semester |
| Module frequency | yearly |
| Module capacity | unlimited |
| Reference text | |
| Module level | |
| Type of module | je nach Studiengang Pflicht oder Wahlpflicht |
| Teaching/Learning method | Seminar (2 CP ), practical course (10 CP) |
| Previous knowledge |
| Type of course | Comment | SWS | Frequency | Workload of compulsory attendance |
|---|---|---|---|---|
| Seminar | 2 | 28 | ||
| Practical training | 8 | 112 | ||
| Total module attendance time | 140 h | |||
| Examination | Examination times | Type of examination |
|---|---|---|
| Final exam of module | Announced at the beginning of the course. |
One assessments of examination: Portfolio: Written protocol and contribution to the seminar (seminar presentation) Seminar presentation (25%), written protocol (75 %). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice or the course. |
