mar520 - Main Module Proteomics (Complete module description)

Module label	Main Module Proteomics
Module code	mar520
Credit points	12.0 KP
Workload	360 h
Institute directory	Institute for Chemistry and Biology of the Marine Environment
Applicability of the module	Master's Programme Microbiology (Master) > Mastermodule
Responsible persons	Rabus, Ralf Andreas (module responsibility) Feenders, Christoph (Module counselling) Wöhlbrand, Lars (Module counselling)
Prerequisites
Skills to be acquired in this module	The students are getting directly involved in actual scientific projects in the area of physiological and/or meta-proteomics (under guidance). They - get acquainted with state-of-the-art proteomic concepts and technologies, - know how to write concise scientific protocols, - know how to present/discuss their results in public.
Module contents	Daily lectures introduce the students to theory and concepts of modern proteomics: (i) separation of cellular compartments and protein extraction, (ii) gel-based and -free protein separation, (iii) gel-staining, protein detection and quantification by image analysis, (iv) integrative mass spectrometry-based protein identification, (v) meta-proteomics, vi) focused genomic analysis. Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project. The following sequence of experiments will be conducted: - extraction and quantification of total protein from prepared cell samples (incl. separation of compartments), - protein separation by SDS-PAGE and staining with Coomassie, silver and/or fluorescent dyes, - digital image acquisition and analysis, - manual and/or automated band excision, - protein identification by nanoLC-ESI-MS/MS, - nanoLC-MALDI-coupling and protein identification by MALDI-TOF-MS/MS, - physiological interpretation of predicted protein functions and relevant genomic context.
Recommended reading	Lottspeich - Bioanalytik
Links
Language of instruction	English
Duration (semesters)	1 Semester
Module frequency	annual
Module capacity	unlimited
Reference text
Type of module	Wahlpflicht / Elective
Module level	AC (Aufbaucurriculum / Composition)
Teaching/Learning method	Seminar (2 CP ), practical course (10 CP)
Previous knowledge	Lecture: Physiology and diversity of prokaryotes, lecture: Molecular Microbiology

Type of course	SWS	Frequency	Workload of compulsory attendance
Seminar	2	SuSe	28
Practical training	8	SuSe	112
Total module attendance time			140 h

Examination	Prüfungszeiten	Type of examination
Final exam of module	Announced at the beginning of the course.	One assessments of examination: Portfolio: Written protocol (75%) and contribution to the seminar (seminar presentation, 25%) Active participation: Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice or the course.