|Module label||Main Module Proteomics|
|Credit points||12.0 KP|
|Institute directory||Institute for Chemistry and Biology of the Marine Environment|
|Applicability of the module||
Lecture: Physiology and diversity of prokaryotes
Lecture: Molecular Microbiology
|Skills to be acquired in this module||
The students are getting directly involved in actual scientific projects in the area of physiological and/or meta-proteomics (under guidance). They - get acquainted with state-of-the-art proteomic concepts and technologies, - know how to write concise scientific protocols, - know how to present/discuss their results in public.
Daily lectures introduce the students to theory and concepts of modern proteomics: (i) separation of cellular compartments and protein extraction, (ii) gel-based and -free protein separation, (iii) gel-staining, protein detection and quantification by image analysis, (iv) integrative mass spectrometry-based protein identification, (v) meta-proteomics, and (vi) focused genomic analysis. Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project. The following sequence of experiments will be conducted: - extraction and quantification of total protein from prepared cell samples (incl. separation of compartments), - protein separation by SDS-PAGE and staining with Coomassie, silver and/or fluorescent dyes, - digital image acquisition and analysis, - manual and/or automated band excision, - protein identification by nanoLC-ESI-MS/MS, - nanoLC-MALDI-coupling and protein identification by MALDI-TOF-MS/MS, - Physiological interpretation of predicted protein functions and relevant genomic context.
Lottspeich - Bioanalytik
|Language of instruction||English|
|Duration (semesters)||1 Semester|
|Type of module||je nach Studiengang Pflicht oder Wahlpflicht|
|Teaching/Learning method||Seminar (2 CP ), practical course (10 CP)|
|Type of course||Comment||SWS||Frequency||Workload of compulsory attendance|
|Total module attendance time||140 h|
|Examination||Examination times||Type of examination|
|Final exam of module||
Announced at the beginning of the course.
One assessments of examination: Portfolio: Written protocol and contribution to the seminar (seminar presentation) Seminar presentation (25%), written protocol (75 %). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice or the course.