Lecture: Physiology and diversity of prokaryotes Lecture: Molecular Microbiology

The students are getting directly involved in actual scientific projects in the area of physiological and/or meta-proteomics (under guidance). They - get acquainted with state-of-the-art proteomic concepts and technologies, - know how to write concise scientific protocols, - know how to present/discuss their results in public.

Daily lectures introduce the students to theory and concepts of modern proteomics: (i) separation of cellular compartments and protein extraction, (ii) gel-based and -free protein separation, (iii) gel-staining, protein detection and quantification by image analysis, (iv) integrative mass spectrometry-based protein identification, (v) meta-proteomics, and (vi) focused genomic analysis. Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project. The following sequence of experiments will be conducted: - extraction and quantification of total protein from prepared cell samples (incl. separation of compartments), - protein separation by SDS-PAGE and staining with Coomassie, silver and/or fluorescent dyes, - digital image acquisition and analysis, - manual and/or automated band excision, - protein identification by nanoLC-ESI-MS/MS, - nanoLC-MALDI-coupling and protein identification by MALDI-TOF-MS/MS, - Physiological interpretation of predicted protein functions and relevant genomic context.

Lottspeich - Bioanalytik

Announced at the beginning of the course.

One assessments of examination: Portfolio: Written protocol and contribution to the seminar (seminar presentation) Seminar presentation (25%), written protocol (75 %). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice or the course