mar520 - Main Module Proteomics (Vollständige Modulbeschreibung)

mar520 - Main Module Proteomics (Vollständige Modulbeschreibung)

Originalfassung Englisch PDF Download
Modulbezeichnung Main Module Proteomics
Modulkürzel mar520
Kreditpunkte 12.0 KP
Workload 360 h
Einrichtungsverzeichnis Institut für Chemie und Biologie des Meeres (ICBM)
Verwendbarkeit des Moduls
  • Master Microbiology (Master) > Mastermodule
Zuständige Personen
  • Rabus, Ralf Andreas (Modulverantwortung)
  • Feenders, Christoph (Modulberatung)
  • Wöhlbrand, Lars (Modulberatung)
Teilnahmevoraussetzungen
Kompetenzziele
The students are getting directly involved in actual scientific projects in the area of physiological and/or meta-proteomics (under guidance).
They
- get acquainted with state-of-the-art proteomic concepts and technologies,
- know how to write concise scientific protocols,
- know how to present/discuss their results in public.
Modulinhalte
Daily lectures introduce the students to theory and concepts of modern proteomics:
(i) separation of cellular compartments and protein extraction,
(ii) gel-based and -free protein separation,
(iii) gel-staining, protein detection and quantification by image analysis,
(iv) integrative mass spectrometry-based protein identification,
(v) meta-proteomics,
(vi) focused genomic analysis.
Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project.
The following sequence of experiments will be conducted:
- extraction and quantification of total protein from prepared cell samples (incl. separation of compartments),
- protein separation by SDS-PAGE and staining with Coomassie, silver and/or fluorescent dyes,
- digital image acquisition and analysis,
- manual and/or automated band excision,
- protein identification by nanoLC-ESI-MS/MS,
- nanoLC-MALDI-coupling and protein identification by MALDI-TOF-MS/MS,
- physiological interpretation of predicted protein functions and relevant genomic context.
Literaturempfehlungen
Lottspeich - Bioanalytik
Links
Unterrichtssprache Englisch
Dauer in Semestern 1 Semester
Angebotsrhythmus Modul annual
Aufnahmekapazität Modul unbegrenzt
Hinweise
Modulart Wahlpflicht / Elective
Modullevel AC (Aufbaucurriculum / Composition)
Lehr-/Lernform Seminar (2 CP ), practical course (10 CP)
Vorkenntnisse Lecture: Physiology and diversity of prokaryotes, lecture: Molecular Microbiology
Lehrveranstaltungsform Kommentar SWS Angebotsrhythmus Workload Präsenz
Seminar 2 SoSe 28
Praktikum 8 SoSe 112
Präsenzzeit Modul insgesamt 140 h
Prüfung Prüfungszeiten Prüfungsform
Gesamtmodul
Announced at the beginning of the course.
One assessments of examination:
Portfolio: Written protocol (75%) and contribution to the seminar (seminar presentation, 25%)

Active participation: Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice or the course.