Stud.IP Uni Oldenburg
Universität Oldenburg
04.07.2020 02:13:05
mar520 - Main Module Proteomics (Vollständige Modulbeschreibung)
Originalfassung Englisch PDF Download
Modulbezeichnung Main Module Proteomics
Modulcode mar520
Kreditpunkte 12.0 KP
Workload 360 h
Fachbereich/Institut Institut für Chemie und Biologie des Meeres (ICBM)
Verwendet in Studiengängen
  • Master Microbiology (Master) > Mastermodule
Ansprechpartner/-in
Modulverantwortung
Modulberatung
Teilnahmevoraussetzungen
Lecture: Physiology and diversity of prokaryotes
Lecture: Molecular Microbiology
Kompetenzziele
The students are getting directly involved in actual scientific projects in the area of physiological and/or meta-proteomics (under guidance). They
  • get acquainted with state-of-the-art proteomic concepts and technologies,
  • know how to write concise scientific protocols,
  • know how to present/discuss their results in public.
Modulinhalte
Functional proteomics: Daily lectures introduce the students to theory and concepts of modern proteomics: (i) separation of cellular compartments and protein extraction, (ii) gel-based and -free protein separation, (iii) gel-staining, protein detection and quantification by image analysis, (iv) integrative mass spectrometry-based protein identification, (v) meta-proteomics, and (vi) focused genomic analysis. Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project. The following sequence of experiments will be conducted:
  • extraction and quantification of total protein from prepared cell samples (incl. separation of compartments),
  • protein separation by SDS-PAGE and staining with Coomassie, silver and/or fluorescent dyes,
  • digital image acquisition and analysis,
  • manual and/or automated band excision,
  • protein identification by nanoLC-ESI-MS/MS,
  • nanoLC-MALDI-coupling and protein identification by MALDI-TOF-MS/MS,
  • Physiological interpretation of predicted protein functions and relevant genomic context.
Literaturempfehlungen
Lottspeich - Bioanalytik
Links
Unterrichtssprache Englisch
Dauer in Semestern 1 Semester
Angebotsrhythmus Modul jährlich
Aufnahmekapazität Modul unbegrenzt
Hinweise
12 CP | SE; PR | 2. FS | Rabus
Modullevel ---
Modulart je nach Studiengang Pflicht oder Wahlpflicht
Lern-/Lehrform / Type of program Seminar (2 CP ), practical course (10 CP)
Vorkenntnisse / Previous knowledge
Lehrveranstaltungsform Kommentar SWS Angebotsrhythmus Workload Präsenzzeit
Seminar 2.00 28 h
Praktikum 8.00 112 h
Präsenzzeit Modul insgesamt 140 h
Prüfung Prüfungszeiten Prüfungsform
Gesamtmodul
Announced at the beginning of the course.
One assessments of examination: Portfolio: Written protocol and contribution to the seminar (seminar presentation)

Seminar presentation (25%), written protocol (75 %). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice or the course