mar520 - Main Module Proteomics (Vollständige Modulbeschreibung)

The students are getting directly involved in actual scientific projects in the area of physiological and/or meta-proteomics (under guidance).
They
- get acquainted with state-of-the-art proteomic concepts and technologies,
- know how to write concise scientific protocols,
- know how to present/discuss their results in public.

Daily lectures introduce the students to theory and concepts of modern proteomics:
(i) separation of cellular compartments and protein extraction,
(ii) gel-based and -free protein separation,
(iii) gel-staining, protein detection and quantification by image analysis,
(iv) integrative mass spectrometry-based protein identification,
(v) meta-proteomics,
(vi) focused genomic analysis.
Each student will prepare a seminar presentation on selected publications relevant for the actual scientific project.
The following sequence of experiments will be conducted:
- extraction and quantification of total protein from prepared cell samples (incl. separation of compartments),
- protein separation by SDS-PAGE and staining with Coomassie, silver and/or fluorescent dyes,
- digital image acquisition and analysis,
- manual and/or automated band excision,
- protein identification by nanoLC-ESI-MS/MS,
- nanoLC-MALDI-coupling and protein identification by MALDI-TOF-MS/MS,
- physiological interpretation of predicted protein functions and relevant genomic context.

Lottspeich - Bioanalytik

Announced at the beginning of the course.

One assessments of examination:
Portfolio: Written protocol (75%) and contribution to the seminar (seminar presentation, 25%)

Active participation: Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice or the course.