| Module label | Profile Module Methods in Aquatic Microbial Ecology |
| Module abbreviation | mar600 |
| Credit points | 6.0 KP |
| Workload | 180 h |
| Institute directory | Institute for Chemistry and Biology of the Marine Environment |
| Applicability of the module |
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| Responsible persons |
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| Prerequisites | For the practical course lecture: Methods in Aquatic Microbial Ecology |
| Skills to be acquired in this module | Skills to be acquired in this module The students learn to… · analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC). · determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyze these data by image analysis. · extract bacterial DNA from water and sediment samples. · amplify bacterial genes by specific primers and PCR. · assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing. · present and discuss scientific results. · write a scientific protocol. The students gain competences in: · Understanding how to analyze dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches. · How to assess the abundance of aquatic bacterial communities by state of the art approaches. · Analyzing the composition of bacterial communities by PCR-based culture-independent approaches. |
| Module contents | The course starts with a lecture introducing basic issues of aquatic microbial ecology with an emphasis on methodological aspects. This lecture is completed before the practical work starts. During the practical course of a block of two weeks the participants carry out analyses and experiments on: The main emphasis is on analyses and approaches of bacterial communities in the water column. |
| Recommended reading | Lecture notes, available on Stud.IP |
| Links | |
| Language of instruction | English |
| Duration (semesters) | 1 Semester |
| Module frequency | annuale |
| Module capacity | unlimited |
| Module level | |
| Type of module | Wahlpflicht / Elective |
| Teaching/Learning method | Block course, 2 weeks; practical course (4 SPPW) and seminar (1 SPPW) |
| Previous knowledge | For the practical course lecture: Methods in Aquatic Microbial Ecology |
| Type of course | Comment | SWS | Frequency | Workload of compulsory attendance |
|---|---|---|---|---|
| Seminar | 1 | 14 | ||
| Exercises | 4 | -- | 56 | |
| Total module attendance time | 70 h | |||
| Examination | Examination times | Type of examination |
|---|---|---|
| Final exam of module | Will be announced during the course |
One assessment of examination: Portfolio (seminar presentation, written protocol) Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.) |
