Module label | Profile Module Methods in Aquatic Microbial Ecology |
Modulkürzel | mar600 |
Credit points | 6.0 KP |
Workload | 180 h |
Institute directory | Institute for Chemistry and Biology of the Marine Environment |
Verwendbarkeit des Moduls |
|
Zuständige Personen |
Simon, Meinhard (Module responsibility)
Brinkhoff, Thorsten Henning (Module counselling)
|
Prerequisites | For the practical course lecture: Methods in Aquatic Microbial Ecology |
Skills to be acquired in this module | Skills to be acquired in this module The students learn to… · analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC). · determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyze these data by image analysis. · extract bacterial DNA from water and sediment samples. · amplify bacterial genes by specific primers and PCR. · assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing. · present and discuss scientific results. · write a scientific protocol. The students gain competences in: · Understanding how to analyze dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches. · How to assess the abundance of aquatic bacterial communities by state of the art approaches. · Analyzing the composition of bacterial communities by PCR-based culture-independent approaches. |
Module contents | The course starts with a lecture introducing basic issues of aquatic microbial ecology with an emphasis on methodological aspects. This lecture is completed before the practical work starts. · the abundance of bacterial communities in aquatic systems · the composition of bacterial communities in environmental samples by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA targeted gene fragments. |
Literaturempfehlungen | Lecture notes, available on Stud.IP |
Links | |
Language of instruction | English |
Duration (semesters) | 1 Semester |
Module frequency | jährlich |
Module capacity | unlimited |
Modullevel / module level | |
Modulart / typ of module | Wahlpflicht / Elective |
Lehr-/Lernform / Teaching/Learning method | Block course, 2 weeks; practical course (4 SPPW) and seminar (1 SPPW) |
Vorkenntnisse / Previous knowledge | For the practical course lecture: Methods in Aquatic Microbial Ecology |
Form of instruction | Comment | SWS | Frequency | Workload of compulsory attendance |
---|---|---|---|---|
Seminar | 1 | 14 | ||
Practical training | 4 | 56 | ||
Präsenzzeit Modul insgesamt | 70 h |
Examination | Prüfungszeiten | Type of examination |
---|---|---|
Final exam of module | Will be announced during the course |
One assessment of examination: Portfolio (seminar presentation, written protocol) Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.) |