gsw150 - Research Project Molecular Biomedicine (Course overview)

Department of Human Medicine

15 KP

all teachers of the curriculum (module counselling, authorized examiners)

Module components

Semester courses Wintersemester 2022/2023

Examination

Seminar

6.03.614 - Human Genetics - Developing therapies to treat splice defect
- Christoph Jüschke
The course times are not decided yet.
6.03.615 - Human Genetics - Mutation identification, pathogenic mechanisms and therapy development
- Prof. Dr. John Neidhardt
The course times are not decided yet.
6.03.616 - Human Genetics - Severe diseases of the cilium: signal transduction and treatment options
- Christoph Jüschke
- Prof. Dr. John Neidhardt
The course times are not decided yet.
6.03.617 - Human Genetics - Transcriptome and Exome analyses in neuronal and neurosensory diseases
- Christoph Jüschke
- Prof. Dr. John Neidhardt
The course times are not decided yet.
6.03.630 - Project: Biochemistry - Protein function in neurosensory systems
- Prof. Dr. Karl-Wilhelm Koch, Dipl.-Chem.
The course times are not decided yet.
The research project will be carried out in the lab of the Biochemistry group. Please contact Prof. Koch for individual timing .
6.03.660 - Neurogenetics - Structure-function analyses of the potassium chloride cotransporter KCC2
- PD Dr. Anna-Maria Hartmann
The course times are not decided yet.
hier können Praxismodule (BA) und Research Module (MA), sowie BA und MA Arbeiten bei mir belegt werden.
6.03.661A - Neurogenetics - Evolution of the auditory system - project A
- Dr. rer. nat. Maike Claußen
The course times are not decided yet.
6.03.661B - Neurogenetics - Evolution of the auditory system - project B
- Dr. Lena Ebbers
The course times are not decided yet.
6.03.662A - Neurogenetics - Analysis of mouse models for deafness - project A
- Dr. rer. nat. Maike Claußen
The course times are not decided yet.
6.03.662B - Neurogenetics - Analysis of mouse models for deafness - project B
- Dr. Lena Ebbers
The course times are not decided yet.
6.03.670 - RM_Visual Neuroscience - Retinal Anatomy
- Prof. Dr. Martin Greschner
- Christian Puller
The course times are not decided yet.
6.03.671 - RM_Visual Neuroscience - Physiology / Data analysis
- Dr. rer. nat. Malte Ahlers
- Prof. Dr. Martin Greschner
- Christian Puller
The course times are not decided yet.
6.03.676 - RM_Visual Neuroscience - Molecular and cellular basis of regeneration in the peripheral nervous system
- Dr. Patrick Dömer
The course times are not decided yet.
6.03.680 - Neurosensorics - Vertebrate retina: Immunohistochemistry, intracellular dye injections, microscopy and image analysis
- Prof. Dr. Karin Dedek
The course times are not decided yet.
joint pre-meeting with all other research modules (one week before the semester starts)
6.03.685 - Anatomy - Molecular and cellular mechanisms of neuronal differentiation
- Prof. Dr. Anja Bräuer
- Dr. rer. nat. Nicola Brandt
The course times are not decided yet.
6.04.150.001 - Gynaecology and Obstetrics – Immunological and cellular mechanisms of gynaecological disorders
- Dr. Lena Dübbel
The course times are not decided yet.
Please contact Dr. Lena Dübbel for individual timing and information (during office hours or by e-mail).
6.04.150.002 - Ophthalmology – Molecular and cellular mechanisms in regenerative Medicine
- PD Dr. Sonja Mertsch
The course times are not decided yet.
Please contact the lecturer for further information.
6.04.150.003 - Immunology - Anti-inflammatory drugs from bacteria and their potential application in the treatment of psoriasis
- Prof. Dr. Karin Loser
- Dr. rer. nat. Nadine Mykicki
The course times are not decided yet.
Practical part: During infection, bacteria, such as Yersinia enterocolitica, can actively translocate anti-inflammatory proteins into the host cell cytoplasm to suppress the host immune response. These proteins can be produced recombinantly and are to be formulated as drugs for therapy. For this purpose, the mouse model of imiquimod-induced psoriasis-like skin inflammation was used, and the animals were treated with the bacterial compounds. After the efficacy has been proven based on the reduction in disease severity, the mechanism of action shall be analyzed in detail. For this purpose, the induction of apoptosis, the inflammasome activation, cytokine production and NF-B activation or the effect of the proteins on the integrity of the epithelial barrier will be analyzed within this research module. Methods such as multicolor immunofluorescence, multiplex assays for cytokine quantification and characterization of signaling cascades, RNA sequencing or kinome analysis will be used. Theoretical part (seminar): During the research module, the pathomechanisms of chronic inflammatory skin diseases will be discussed, with a specific focus on the involvement of the immune system in the development of psoriasis vulgaris. At the same time, various possibilities how bacterial pathogens evade host immune defenses will be presented. These possibilities include the production of cell-penetrating peptides (CPEs) with anti-inflammatory properties, which can be developed as agents for therapy (drugs-from-bugs concept).
6.04.150.005 - Immunology - Novel immunomodulatory tripeptides for the treatment of inflammation
- Prof. Dr. Karin Loser
- Dr. rer. nat. Nadine Mykicki
The course times are not decided yet.
Objectives The endogenous opioid system plays an important role in the pathogenesis of inflammatory disorders and opioid receptor agonists, mainly agonists of the kappa-opioid receptor (KOR), are able to regulate cytokine production, immune cell activation and migration. Therefore, activation of KOR could represent a novel promising approach for the treatment of (sterile) inflammation. Therefore, we developed quinoline- and quinoxaline-based KOR agonists with high affinity, selectivity, and full agonistic activity. These KOR agonists reduced the production of pro-inflammatory cytokines (e.g., IL-1, IL-6, TNF, IFN-y) as well as immune cell proliferation upon activation. The observed effects were mediated via KOR signalling, since off-target effects were excluded by using KOR deficient mouse mutants. Now we intend to develop new KOR agonists based on novel scaffolds. In particular, diverse substituents in 4- or 5-position of the perhydroquinoline system allow versatile modifications allowing fine-tuning of pharmacodynamic and pharmacokinetic properties. These novel KOR agonists will be used to better understand the relevance of KOR signalling in inflammatory diseases. Hence, the effect of KOR agonists on phenotype, function and migratory activity will be analyzed in primary mouse and human immune cells by multicolor flow cytometry, multiplex bead assays or impedance-based measurements using the xCELLigence®-system). Moreover, the metabolic activity and transcription profile of immune cells treated with KOR agonists shall be assessed using the Seahorse® technology and (single cell) RNA sequencing.
6.04.150.006 - Perioperative Inflammation & Infection: Basic research in cardiovascular diseases - Heartfailure
- Lisa Hasselbach
- Prof. Dr. Gregor Theilmeier
The course times are not decided yet.
6.04.150.007 - Perioperative Inflammation & Infection: Basic research in cardiovascular diseases - Coronary artery disease
- Prof. Dr. Gregor Theilmeier
The course times are not decided yet.
6.04.150.008 - Pharmacology & Toxicology – Expression of Protease activated receptors in Vulva and Cervix carcinoma
- Dr. rer. nat. Ulrike Meyer
- Prof. Dr. med. Bernhard Rauch
The course times are not decided yet.
Please contact Dr. Ulrike Meyer for individual timing and further information

Project (Individuelles Forschungsprojekt)

6.03.614 - Human Genetics - Developing therapies to treat splice defect
- Christoph Jüschke
The course times are not decided yet.
6.03.615 - Human Genetics - Mutation identification, pathogenic mechanisms and therapy development
- Prof. Dr. John Neidhardt
The course times are not decided yet.
6.03.616 - Human Genetics - Severe diseases of the cilium: signal transduction and treatment options
- Christoph Jüschke
- Prof. Dr. John Neidhardt
The course times are not decided yet.
6.03.617 - Human Genetics - Transcriptome and Exome analyses in neuronal and neurosensory diseases
- Christoph Jüschke
- Prof. Dr. John Neidhardt
The course times are not decided yet.
6.03.630 - Project: Biochemistry - Protein function in neurosensory systems
- Prof. Dr. Karl-Wilhelm Koch, Dipl.-Chem.
The course times are not decided yet.
The research project will be carried out in the lab of the Biochemistry group. Please contact Prof. Koch for individual timing .
6.03.660 - Neurogenetics - Structure-function analyses of the potassium chloride cotransporter KCC2
- PD Dr. Anna-Maria Hartmann
The course times are not decided yet.
hier können Praxismodule (BA) und Research Module (MA), sowie BA und MA Arbeiten bei mir belegt werden.
6.03.661A - Neurogenetics - Evolution of the auditory system - project A
- Dr. rer. nat. Maike Claußen
The course times are not decided yet.
6.03.661B - Neurogenetics - Evolution of the auditory system - project B
- Dr. Lena Ebbers
The course times are not decided yet.
6.03.662A - Neurogenetics - Analysis of mouse models for deafness - project A
- Dr. rer. nat. Maike Claußen
The course times are not decided yet.
6.03.662B - Neurogenetics - Analysis of mouse models for deafness - project B
- Dr. Lena Ebbers
The course times are not decided yet.
6.03.670 - RM_Visual Neuroscience - Retinal Anatomy
- Prof. Dr. Martin Greschner
- Christian Puller
The course times are not decided yet.
6.03.671 - RM_Visual Neuroscience - Physiology / Data analysis
- Dr. rer. nat. Malte Ahlers
- Prof. Dr. Martin Greschner
- Christian Puller
The course times are not decided yet.
6.03.676 - RM_Visual Neuroscience - Molecular and cellular basis of regeneration in the peripheral nervous system
- Dr. Patrick Dömer
The course times are not decided yet.
6.03.680 - Neurosensorics - Vertebrate retina: Immunohistochemistry, intracellular dye injections, microscopy and image analysis
- Prof. Dr. Karin Dedek
The course times are not decided yet.
joint pre-meeting with all other research modules (one week before the semester starts)
6.03.685 - Anatomy - Molecular and cellular mechanisms of neuronal differentiation
- Prof. Dr. Anja Bräuer
- Dr. rer. nat. Nicola Brandt
The course times are not decided yet.
6.04.150.001 - Gynaecology and Obstetrics – Immunological and cellular mechanisms of gynaecological disorders
- Dr. Lena Dübbel
The course times are not decided yet.
Please contact Dr. Lena Dübbel for individual timing and information (during office hours or by e-mail).
6.04.150.002 - Ophthalmology – Molecular and cellular mechanisms in regenerative Medicine
- PD Dr. Sonja Mertsch
The course times are not decided yet.
Please contact the lecturer for further information.
6.04.150.003 - Immunology - Anti-inflammatory drugs from bacteria and their potential application in the treatment of psoriasis
- Prof. Dr. Karin Loser
- Dr. rer. nat. Nadine Mykicki
The course times are not decided yet.
Practical part: During infection, bacteria, such as Yersinia enterocolitica, can actively translocate anti-inflammatory proteins into the host cell cytoplasm to suppress the host immune response. These proteins can be produced recombinantly and are to be formulated as drugs for therapy. For this purpose, the mouse model of imiquimod-induced psoriasis-like skin inflammation was used, and the animals were treated with the bacterial compounds. After the efficacy has been proven based on the reduction in disease severity, the mechanism of action shall be analyzed in detail. For this purpose, the induction of apoptosis, the inflammasome activation, cytokine production and NF-B activation or the effect of the proteins on the integrity of the epithelial barrier will be analyzed within this research module. Methods such as multicolor immunofluorescence, multiplex assays for cytokine quantification and characterization of signaling cascades, RNA sequencing or kinome analysis will be used. Theoretical part (seminar): During the research module, the pathomechanisms of chronic inflammatory skin diseases will be discussed, with a specific focus on the involvement of the immune system in the development of psoriasis vulgaris. At the same time, various possibilities how bacterial pathogens evade host immune defenses will be presented. These possibilities include the production of cell-penetrating peptides (CPEs) with anti-inflammatory properties, which can be developed as agents for therapy (drugs-from-bugs concept).
6.04.150.005 - Immunology - Novel immunomodulatory tripeptides for the treatment of inflammation
- Prof. Dr. Karin Loser
- Dr. rer. nat. Nadine Mykicki
The course times are not decided yet.
Objectives The endogenous opioid system plays an important role in the pathogenesis of inflammatory disorders and opioid receptor agonists, mainly agonists of the kappa-opioid receptor (KOR), are able to regulate cytokine production, immune cell activation and migration. Therefore, activation of KOR could represent a novel promising approach for the treatment of (sterile) inflammation. Therefore, we developed quinoline- and quinoxaline-based KOR agonists with high affinity, selectivity, and full agonistic activity. These KOR agonists reduced the production of pro-inflammatory cytokines (e.g., IL-1, IL-6, TNF, IFN-y) as well as immune cell proliferation upon activation. The observed effects were mediated via KOR signalling, since off-target effects were excluded by using KOR deficient mouse mutants. Now we intend to develop new KOR agonists based on novel scaffolds. In particular, diverse substituents in 4- or 5-position of the perhydroquinoline system allow versatile modifications allowing fine-tuning of pharmacodynamic and pharmacokinetic properties. These novel KOR agonists will be used to better understand the relevance of KOR signalling in inflammatory diseases. Hence, the effect of KOR agonists on phenotype, function and migratory activity will be analyzed in primary mouse and human immune cells by multicolor flow cytometry, multiplex bead assays or impedance-based measurements using the xCELLigence®-system). Moreover, the metabolic activity and transcription profile of immune cells treated with KOR agonists shall be assessed using the Seahorse® technology and (single cell) RNA sequencing.
6.04.150.006 - Perioperative Inflammation & Infection: Basic research in cardiovascular diseases - Heartfailure
- Lisa Hasselbach
- Prof. Dr. Gregor Theilmeier
The course times are not decided yet.
6.04.150.007 - Perioperative Inflammation & Infection: Basic research in cardiovascular diseases - Coronary artery disease
- Prof. Dr. Gregor Theilmeier
The course times are not decided yet.
6.04.150.008 - Pharmacology & Toxicology – Expression of Protease activated receptors in Vulva and Cervix carcinoma
- Dr. rer. nat. Ulrike Meyer
- Prof. Dr. med. Bernhard Rauch
The course times are not decided yet.
Please contact Dr. Ulrike Meyer for individual timing and further information

Hinweise zum Modul

Prerequisites	as defined in the admission and examination regulations
Module examination	graded: project report ungraded: participation in seminar and 30 min. presentation
Skills to be acquired in this module	Competencies: ++ deepened biological and / or clinical expertise ++ deepened knowledge of biological working methods and / or clinical diagnostics ++ data analysis skills + interdisciplinary thinking ++ critical and analytical thinking ++ independent searching and knowledge of scientific literature ++ ability to perform independent biological research ++ data presentation and discussion (written and spoken) + team work + ethics and professional behaviour + project and time management