Stud.IP Uni Oldenburg
Universität Oldenburg
16.01.2021 19:47:25
mar600 - Profile Module Methods in Aquatic Microbial Ecology (Veranstaltungsübersicht)
Institut für Chemie und Biologie des Meeres (ICBM) 6 KP
  • Meinhard Simon
  • Thorsten Henning Brinkhoff
Modulteile Semesterveranstaltungen Wintersemester 2020/2021 Prüfungsleistung
  • Uneingeschränkter Zugang 5.08.4632 - Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie headache
    • Apl. Prof. Dr. Thorsten Henning Brinkhoff
    • Dr. Helge-Ansgar Giebel
    • Dr. Liliana Cristina Moraru
    • Prof. Dr. Meinhard Simon

    Termine am Montag. 30.11.20 - Freitag. 04.12.20, Montag. 07.12.20 - Freitag. 11.12.20 08:30 - 17:00
    Voraussetzung: VL Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie Preconditions: Lecture Methods in Aquatic Microbial Ecology

Hinweise zum Modul
For the practical course lecture: Methods in Aquatic Microbial Ecology
Will be announced during the course.
Prüfungsleistung Modul
One assessment of examination: Portfolio (seminar presentation, written protocol) Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)
The students learn to: - Analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC), organic carbon by TOC and POC/PON analyser and the composition of the pool of dissolved organic matter by Fourier-Transform Ion Cyclotron Resonance Mass spectrometry (FT-ICR-MS). - Determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyse these data by image analysis. - Extract bacterial DNA from water and sediment samples. - to amplify bacterial genes by specific primers and PCR. - Assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis. - present and discuss scientific results - write a scientific protocol - The students gain competences in: - Understanding how to analyse dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches. - How to assess the abundance of aquatic bacterial communities by state of the art approaches. - Analyzing the composition of bacterial communities by PCR-based culture-independent approaches.