Stud.IP Uni Oldenburg
Universität Oldenburg
25.09.2020 03:21:22
mar600 - Profile Module Methods in Aquatic Microbial Ecology (Veranstaltungsübersicht)
Institut für Chemie und Biologie des Meeres (ICBM) 6 KP
Modulverantwortung
  • Meinhard Simon
Modulberatung
  • Thorsten Henning Brinkhoff
Modulteile Semesterveranstaltungen Wintersemester 2020/2021 Prüfungsleistung
Seminar
Praktikum
  • Uneingeschränkter Zugang 5.08.4632 - Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie headache
    • Apl. Prof. Dr. Thorsten Henning Brinkhoff
    • Dr. Helge-Ansgar Giebel
    • Dr. Liliana Cristina Moraru
    • Prof. Dr. Meinhard Simon

    Termine am Montag. 30.11.20 - Freitag. 04.12.20, Montag. 07.12.20 - Freitag. 11.12.20 08:30 - 17:00
    Voraussetzung: VL Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie Preconditions: Lecture Methods in Aquatic Microbial Ecology

Hinweise zum Modul
Teilnahmevoraussetzungen
For the practical course lecture: Methods in Aquatic Microbial Ecology
Prüfungsleistung Modul
One assessment of examination:
Portfolio (seminar presentation, written protocol)

Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)
Kompetenzziele
The students learn to:
- Analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC), organic carbon by TOC and POC/PON analyser and the composition of the pool of dissolved organic matter by Fourier-Transform Ion Cyclotron Resonance Mass spectrometry (FT-ICR-MS).
- Determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyse these data by image analysis.
- Extract bacterial DNA from water and sediment samples.
- to amplify bacterial genes by specific primers and PCR.
- Assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis.
- present and discuss scientific results
- write a scientific protocol
- The students gain competences in:
- Understanding how to analyse dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.
- How to assess the abundance of aquatic bacterial communities by state of the art approaches.
- Analyzing the composition of bacterial communities by PCR-based culture-independent approaches.