mar600 - Profile Module Methods in Aquatic Microbial Ecology (Veranstaltungsübersicht)

Will be announced during the course.

One assessment of examination:
Portfolio: Protocol (100 %), seminar presentation (no mark)

Active participation: Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.
 

Skills to be acquired in this module

The students learn to…
- analyse bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC).
- determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyse these data by image analysis.
- extract bacterial DNA from water and sediment samples.
- amplify bacterial genes by specific primers and PCR.
- assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing.
- present and discuss scientific results.
- write a scientific protocol.

The students gain competences in:
- Understanding how to analyse dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.
- How to assess the abundance of aquatic bacterial communities by state of the art approaches.
- Analysing the composition of bacterial communities by PCR-based culture-independent approaches.