mar600 - Profile Module Methods in Aquatic Microbial Ecology (Course overview)

mar600 - Profile Module Methods in Aquatic Microbial Ecology (Course overview)

Institute for Chemistry and Biology of the Marine Environment 6 KP
Module components Semester courses Examination
Seminar
  • No access 5.08.4631 - Methods in Aquatic Microbial Ecology Show lecturers
    • Prof. Dr. Meinhard Simon
    • Prof. Dr. Thorsten Henning Brinkhoff
    • Dr. Matthias Wietz, Ph.D.

    Dates on Monday, 14.11.2022 - Tuesday, 15.11.2022, Thursday, 17.11.2022, Monday, 21.11.2022 - Tuesday, 22.11.2022, Thursday, 24.11.2022 14:15 - 15:45
Exercises
  • No access 5.08.4632 - Methods in Aquatic Microbial Ecology Show lecturers
    • Prof. Dr. Thorsten Henning Brinkhoff
    • Prof. Dr. Meinhard Simon
    • Dr. Matthias Wietz, Ph.D.
    • Jana Stone

    Dates on Monday, 28.11.2022 - Friday, 02.12.2022, Monday, 05.12.2022 - Friday, 09.12.2022 08:30 - 17:00
Hinweise zum Modul
Prüfungszeiten
Will be announced during the course
Module examination
One assessment of examination:
Portfolio: Protocol (100 %), seminar presentation (no mark)

Active participation: Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.
Skills to be acquired in this module

Skills to be acquired in this module

The students learn to…

·       analyse bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC).

·       determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyse these data by image analysis.

·       extract bacterial DNA from water and sediment samples.

·       amplify bacterial genes by specific primers and PCR.

·       assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing.

·       present and discuss scientific results.

·       write a scientific protocol.

The students gain competences in:

·       Understanding how to analyse dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.

·       How to assess the abundance of aquatic bacterial communities by state of the art approaches.

·       Analysing the composition of bacterial communities by PCR-based culture-independent approaches.