Stud.IP Uni Oldenburg
University of Oldenburg
09.08.2022 03:19:20
mar600 - Profile Module Methods in Aquatic Microbial Ecology (Course overview)
Institute for Chemistry and Biology of the Marine Environment 6 KP
Module components Semester courses Wintersemester 2021/2022 Examination
Seminar
  • No access 5.08.4631 - Methods in Aquatic Microbial Ecology Show lecturers
    • Prof. Dr. Meinhard Simon
    • Apl. Prof. Dr. Thorsten Henning Brinkhoff
    • Dr. Liliana Cristina Moraru

    Dates on Monday. 15.11.21 - Tuesday. 16.11.21, Thursday. 18.11.21, Monday. 22.11.21 - Tuesday. 23.11.21, Thursday. 25.11.21 14:15 - 15:45
Practical training
  • No access 5.08.4632 - Methods in Aquatic Microbial Ecology Show lecturers
    • Apl. Prof. Dr. Thorsten Henning Brinkhoff
    • Dr. Helge-Ansgar Giebel
    • Dr. Liliana Cristina Moraru
    • Prof. Dr. Meinhard Simon

    Dates on Monday. 29.11.21 - Friday. 03.12.21 08:30 - 17:00, Monday. 06.12.21 08:15 - 11:45, Monday. 06.12.21 - Wednesday. 08.12.21 08: ...(more), Location: W15 1-146, W15 0-023, ((Labore der AG))
    Voraussetzung: VL Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie 5.08.4631 Preconditions: Lecture Methods in Aquatic Microbial Ecology 5.08.4631

Notes for the module
Prerequisites
For the practical course lecture: Methods in Aquatic Microbial Ecology
Time of examination
Will be announced during the course
Module examination
One assessment of examination: Portfolio (seminar presentation, written protocol)

Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)
Skills to be acquired in this module

Skills to be acquired in this module

The students learn to…

·       analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC).

·       determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyze these data by image analysis.

·       extract bacterial DNA from water and sediment samples.

·       amplify bacterial genes by specific primers and PCR.

·       assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing.

·       present and discuss scientific results.

·       write a scientific protocol.

The students gain competences in:

·       Understanding how to analyze dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.

·       How to assess the abundance of aquatic bacterial communities by state of the art approaches.

·       Analyzing the composition of bacterial communities by PCR-based culture-independent approaches.