mar600 - Profile Module Methods in Aquatic Microbial Ecology (Veranstaltungsübersicht)

mar600 - Profile Module Methods in Aquatic Microbial Ecology (Veranstaltungsübersicht)

Institut für Chemie und Biologie des Meeres (ICBM) 6 KP
Modulteile Semesterveranstaltungen Wintersemester 2021/2022 Prüfungsleistung
Seminar
Übung
  • Kein Zugang 5.08.4632 - Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie Lehrende anzeigen
    • Prof. Dr. Thorsten Henning Brinkhoff
    • Dr. Helge-Ansgar Giebel
    • Dr. Liliana Cristina Moraru
    • Prof. Dr. Meinhard Simon

    Termine am Montag, 29.11.2021 - Freitag, 03.12.2021 08:30 - 17:00, Montag, 06.12.2021 08:15 - 11:45, Montag, 06.12.2021 - Mittwoch, 08.1 ...(mehr), Ort: W15 1-146, W15 0-023, ((Labore der AG))
    Voraussetzung: VL Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie 5.08.4631 Preconditions: Lecture Methods in Aquatic Microbial Ecology 5.08.4631

Hinweise zum Modul
Teilnahmevoraussetzungen
Prüfungszeiten
Will be announced during the course.
Prüfungsleistung Modul
One assessment of examination:
Portfolio: Protocol (100 %), seminar presentation (no mark)

Active participation: Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.
 
Kompetenzziele

Skills to be acquired in this module

The students learn to…
- analyse bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC).
- determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyse these data by image analysis.
- extract bacterial DNA from water and sediment samples.
- amplify bacterial genes by specific primers and PCR.
- assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing.
- present and discuss scientific results.
- write a scientific protocol.

The students gain competences in:
- Understanding how to analyse dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.
- How to assess the abundance of aquatic bacterial communities by state of the art approaches.
- Analysing the composition of bacterial communities by PCR-based culture-independent approaches.