Stud.IP Uni Oldenburg
Universität Oldenburg
03.12.2021 13:44:26
mar600 - Profile Module Methods in Aquatic Microbial Ecology (Veranstaltungsübersicht)
Institut für Chemie und Biologie des Meeres (ICBM) 6 KP
Modulteile Semesterveranstaltungen Wintersemester 2021/2022 Prüfungsleistung
Seminar
Praktikum
  • Eingeschränkter Zugang 5.08.4632 - Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie Lehrende anzeigen
    • Apl. Prof. Dr. Thorsten Henning Brinkhoff
    • Dr. Helge-Ansgar Giebel
    • Dr. Liliana Cristina Moraru
    • Prof. Dr. Meinhard Simon

    Termine am Montag. 29.11.21 - Freitag. 03.12.21 08:30 - 17:00, Montag. 06.12.21 08:15 - 11:45, Montag. 06.12.21 - Mittwoch. 08.12.21 08: ...(mehr), Ort: W15 1-146, W15 0-023, ((Labore der AG)) (+1 weitere)
    Voraussetzung: VL Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie 5.08.4631 Preconditions: Lecture Methods in Aquatic Microbial Ecology 5.08.4631

Hinweise zum Modul
Teilnahmevoraussetzungen
For the practical course lecture: Methods in Aquatic Microbial Ecology
Prüfungszeiten
Will be announced during the course.
Prüfungsleistung Modul
One assessment of examination: Portfolio (seminar presentation, written protocol)

Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)
Kompetenzziele

Skills to be acquired in this module

The students learn to…

·       analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC).

·       determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyze these data by image analysis.

·       extract bacterial DNA from water and sediment samples.

·       amplify bacterial genes by specific primers and PCR.

·       assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing.

·       present and discuss scientific results.

·       write a scientific protocol.

The students gain competences in:

·       Understanding how to analyze dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.

·       How to assess the abundance of aquatic bacterial communities by state of the art approaches.

·       Analyzing the composition of bacterial communities by PCR-based culture-independent approaches.