|Title||Multi-component bioprinting of artificial skin|
Bioprinting is a group of additive manufacturing (commonly known as 3D printing) technologies aiming at designed assembly of biologically relevant materials in 2D and 3D structures which accomplish one or more biological functions. In the last years, at the Department of Chemistry in cooperation with the European Medical School we have developed a new inkjet pneumatic conveying bioprinting technique. The special advantage of this technique arises from the possibility to use viscous liquids as a bioink. In this way, printing is possible with live cells that can reach high concentrations, approaching 20 x 106 cell per mL.
Human skin is much more than just the sum of its individual components, that include various cell types such as fibroblasts, keratinocytes, Langerhans cells, melanocytes, effector cells and others. It has a complex three-dimensional stratified structure which cannot be reproduced in 2-dimensional cell culture experiments. In this regard, bioprinting offers excellent opportunity to produce such complex system.
In this interdisciplinary work pneumatic conveying bioprinting will be used to produce fibroblasts, and keratinocytes layers. The challenge of this project is to provide structure and stability to the printed layers, consisting of hydrogel scaffolds and live cells. This would require designing appropriate ink composition. Importantly, it has to serve as an artificial extracellular matrix, which over time, has to be substituted with the extracellular matrix produced by the cells. For this purpose, the components of the bioink have to undergo a fast gelation reaction with the substrate and / or with the material which will be delivered in parallel during the printing procedure. In addition, the project work will include chemical analysis with IR spectroscopy and characterization of materials with other techniques, such as scanning electron microscopy as well as fluorescence imaging for monitoring cells.
|Home institution||Department of Chemistry|
|Type of work||practical / application-focused|
|Type of thesis||Master's degree|
|Author||Dr. Izabella Brand|
Biodurkcen Verfahren, Spektrtoskopiesche Techniken
Kooperatinsarbeit mit Fakultät VI
Bachelor in Chemistry