mar570 - Profile Module Introduction to DNA-sequencing and sequence analysis

mar570 - Profile Module Introduction to DNA-sequencing and sequence analysis

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Module label Profile Module Introduction to DNA-sequencing and sequence analysis
Modulkürzel mar570
Credit points 6.0 KP
Workload 180 h
Institute directory Institute for Chemistry and Biology of the Marine Environment
Verwendbarkeit des Moduls
  • Master's Programme Microbiology (Master) >
Zuständige Personen
  • Brinkhoff, Thorsten Henning (module responsibility)
Prerequisites
Skills to be acquired in this module
The students know how to
- sequence DNA by Sanger sequencing
- assemble DNA sequences
- use internet databases for sequence comparison
- use the various facilities of the NCBI database
- analyze bacterial genomes for presence of specific genes
- use Genious for genome analysis
- use ARB, databases and literature data to create phylogenetic trees, design primers and probes
- present and discuss scientific results
- write a scientific protocol 
Module contents
“Introduction into DNA-sequencing and sequence analysis”: The course starts with a lecture on the first two days. During the following days the participants will give seminar talks about different scientific studies for which DNA sequencing was highly relevant. DNA sequencing will be taught in the lab of the working group. Sequence analysis, introduction into the use of various internet databases, the sequence analysis program Genious and the phylogeny programme ARB will be demonstrated by individual use of laptops of the institute.
Literaturempfehlungen
Links
Language of instruction English
Duration (semesters) 1 Semester
Module frequency annual
Module capacity 18
Type of module Wahlpflicht / Elective
Module level AC (Aufbaucurriculum / Composition)
Teaching/Learning method Seminar; practical course
Previous knowledge Lecture during the course
Form of instruction Comment SWS Frequency Workload of compulsory attendance
Seminar 2 SoSe 28
Exercises 4 SoSe 56
Präsenzzeit Modul insgesamt 84 h
Examination Prüfungszeiten Type of examination
Final exam of module
Announced during the course.
One assessment of examination:
Portfolio: Protocol (75 %), seminar presentation (25 %)

Active participation: Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.

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