mar454 - Einführung in die DNA-Sequenzierung und Sequenzanalyse

The students know how to

- manually assemble 16S rRNA gene sequences

- assemble metagenomic data

- use internet databases for sequence comparison

- use the various facilities of the NCBI database

- analyze bacterial genomes for presence of specific genes

- use ARB, databases and literature data

- create phylogenetic trees

- design primers and probes

- use basic BASH 

- use basic High-performance computers

- use basic R data visualization

- present and discuss scientific results

Einführung in die Sequenzierung und Sequenzanalyse

The course starts with a lecture on the first two days. During the following days the participants will give seminar talks about different scientific studies for which DNA sequencing was highly relevant. DNA sequencing will be taught in the lab of the working group. Sequence analysis, introduction into the use of various internet databases, the sequence analysis program Genious and the phylogeny program ARB will be demonstrated by individual use of laptops of the institute.

Will be announced.

Procedure see Stud.IP

Will be announced by the lecturer at the beginning of the course.

1 graded examination

Portfolio (seminar presentation, written protocol) or internship report or presentation or protocol (75 %), seminar presentation (25 %).

Active participation
Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.