mar454 - Introduction to DNA Sequencing and Sequence Analysis

mar454 - Introduction to DNA Sequencing and Sequence Analysis

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Module label Introduction to DNA Sequencing and Sequence Analysis
Modulkürzel mar454
Credit points 6.0 KP
Workload 180 h
Institute directory Institute for Chemistry and Biology of the Marine Environment
Verwendbarkeit des Moduls
  • Master's Programme Marine Environmental Sciences (Master) > Mastermodule
Zuständige Personen
  • Brinkhoff, Thorsten Henning (module responsibility)
  • Garcia, Sarahi Lorena (Module counselling)
Prerequisites
none
Skills to be acquired in this module

The students know how to

- sequence DNA by Sanger sequencing

- assemble DNA sequences

- use internet databases for sequence comparison

- use the various facilities of the NCBI database

- analyze bacterial genomes for presence of specific Genes

- use Genious for genome analysis

- use ARB, databases and literature data

- create phylogenetic trees

- design primers and probes

- present and discuss scientific results

- write a scientific protocol  

Module contents

Einführung in die Sequenzierung und Sequenzanalyse

The course starts with a lecture on the first two days. During the following days the participants will give seminar talks about different scientific studies for which DNA sequencing was highly relevant. DNA sequencing will be taught in the lab of the working group. Sequence analysis, introduction into the use of various internet databases, the sequence analysis program Genious and the phylogeny program ARB will be demonstrated by individual use of laptops of the institute.

Literaturempfehlungen
Will be announced
Links
Languages of instruction German, English
Duration (semesters) 1 Semester
Module frequency annually
Module capacity 12 (
Procedure see StudIP
)
Lehrveranstaltungsform Comment SWS Frequency Workload of compulsory attendance
Seminar 2 SoSe 28
Exercises 4 SoSe 56
Präsenzzeit Modul insgesamt 84 h
Examination Prüfungszeiten Type of examination
Final exam of module

Will be announced by the lecturer at the beginning of the course.

1 graded examination

Portfolio (seminar presentation, written protocol) or internship report or presentation or protocol (75 %), seminar presentation (25 %).

Active participation
Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.


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