For the practical course lecture: Methods in Aquatic Microbial Ecology
Will be announced during the course.
One assessment of examination: Portfolio (seminar presentation, written protocol)
Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)
Skills to be acquired in this module
The students learn to…
·analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC).
·determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyze these data by image analysis.
·extract bacterial DNA from water and sediment samples.
·amplify bacterial genes by specific primers and PCR.
·assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing.
·present and discuss scientific results.
·write a scientific protocol.
The students gain competences in:
·Understanding how to analyze dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.
·How to assess the abundance of aquatic bacterial communities by state of the art approaches.
·Analyzing the composition of bacterial communities by PCR-based culture-independent approaches.