Stud.IP Uni Oldenburg
Universität Oldenburg
19.10.2019 06:17:49
mar600 - Profile Module Methods in Aquatic Microbial Ecology
Institut für Chemie und Biologie des Meeres (ICBM) 6 KP
Modulteile Semesterveranstaltungen Wintersemester 2019/2020 Prüfungsleistung
Seminar
Praktikum
  • Eingeschränkter Zugang 5.08.4632 - Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie headache
    • Apl. Prof. Dr. Thorsten Henning Brinkhoff
    • Dr. Helge-Ansgar Giebel
    • Dr. Liliana Cristina Moraru
    • Prof. Dr. Meinhard Simon
    • Birgit Kürzel
    • Mathias Wolterink
    • Rolf Weinert

    Termine am Montag. 25.11.19 08:30 - 09:00, Montag. 25.11.19 - Freitag. 29.11.19, Montag. 02.12.19 08:30 - 17:00, Dienstag. 03.12.19 08:0 ...(mehr), Ort: W15 1-146, W15 2-234, (Labore der AG)
    Voraussetzung: VL Untersuchungsmethoden in der Aquatischen Mikrobiellen Ökologie Preconditions: Lecture Methods in Aquatic Microbial Ecology

Hinweise zum Modul
Teilnahmevoraussetzungen
For the practical course lecture: Methods in Aquatic Microbial Ecology
Prüfungsleistung Modul
One assessment of examination:
Portfolio (seminar presentation, written protocol)

Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)
Kompetenzziele
The students learn to:
- Analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC), organic carbon by TOC and POC/PON analyser and the composition of the pool of dissolved organic matter by Fourier-Transform Ion Cyclotron Resonance Mass spectrometry (FT-ICR-MS).
- Determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyse these data by image analysis.
- Extract bacterial DNA from water and sediment samples.
- to amplify bacterial genes by specific primers and PCR.
- Assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis.
- present and discuss scientific results
- write a scientific protocol
- The students gain competences in:
- Understanding how to analyse dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.
- How to assess the abundance of aquatic bacterial communities by state of the art approaches.
- Analyzing the composition of bacterial communities by PCR-based culture-independent approaches.

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