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27.10.2021 03:45:30
mar610 - Profile Module Isolation and characterization of microorganisms (Complete module description)
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Module label Profile Module Isolation and characterization of microorganisms
Module code mar610
Credit points 6.0 KP
Workload 180 h
Institute directory Institute for Chemistry and Biology of the Marine Environment
Applicability of the module
  • Master's Programme Microbiology (Master) > Mastermodule
Responsible persons
Könneke, Martin (Module responsibility)
Engelen, Bert (Module counselling)
Prerequisites
Microbial Physiology and diversity (M1)
Skills to be acquired in this module
In this course the students will isolate bacteria and other microorganisms. They will learn classical microbiological techniques as enrichment culture, aseptic work, preparation of liquid and solid media, cultivation under oxic and anoxic condition, on agar plates and in deep agar dilution, description of microbes by techniques as staining, microscopy, microphotography.
Module contents
Isolation and characterization of microorganisms: Seminar
Prior to the laboratory work the participants shall read literature about first isolation, description and current studies on their target organisms and present this and their isolation strategy in the seminar. During the course and at the end, results and a possible molecular identification of isolates will be presented and discussed.

Practical work: Every student prepares media and agar plates required for the isolation of the different target organisms. If pure cultures have been isolated, they should be transferred to long-term storage on agar and in liquid nitrogen. Sampling sites and different stages of the enrichment and isolation are documented by macro- and microphotography and described in the report. Finally, tests to verify purity of the culture and its identification, as well as a phylogenetic analysis are requested.
Reader's advisory
Brock. Biology of Microorganisms / Cypionka, Grundlagen der Mikrobiologie / Drews, G. Mikrobiologisches Praktikum, 1974 / DSMZ catalogue (www.dsmz.de) / Dyer, B.D. A field guide to the bacteria. 2003 /Praktikumsskripte, Uni Göttingen, Uni Konstanz / Reddy, C.A. Methods for general and molecular Microbiology. 2007 / Steinbüchel et al. Mikrobiologisches Praktikum. 2012 / www.microbiogical-garden.net
Links
Language of instruction English
Duration (semesters) 1 Semester
Module frequency jährlich
Module capacity unlimited
Reference text
6 CP | SE; PR | 1. or 3. FS | Cypionka
Modullevel / module level MM (Mastermodul / Master module)
Modulart / typ of module Wahlpflicht / Elective
Lehr-/Lernform / Teaching/Learning method Seminar and laboratory work, twice per week, half a day each
Vorkenntnisse / Previous knowledge Module mar500 including lectures on “Physiology and life modes of prokaryotes” and “Microbial diversity”
Course type Comment SWS Frequency Workload of compulsory attendance
Seminar
1 14
Practical training
4 56
Total time of attendance for the module 70 h
Examination Time of examination Type of examination
Final exam of module
Announced during the course.
One assessment of examination:
Portfolio (seminar presentation, written protocol)

Protocol (100 %), webpage, seminar presentation (no mark). . Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)