mar610 Profile Module Isolation and characterization of microorganisms (Complete module description)

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In this course the students will isolate bacteria and other microorganisms. They will learn classical microbiological techniques as enrichment culture, aseptic work, preparation of liquid and solid media, cultivation under oxic and anoxic condition, on agar plates and in deep agar dilution, description of microbes by techniques as staining, microscopy, microphotography.

Prior to the laboratory work the participants shall read literature and current studies about their target group of microorganisms and develop an enrichment strategy isolation. They will present this and their enrichment strategy in the seminar. During the course and at the end, results and a possible molecular identification of isolates will be presented and discussed.

Practical work: Student prepares media and agar plates required for the enrichment and isolation of the different target organisms in small groups. The enrichment cultures will be monitored over time by measuring various biological and chemical parameters. If pure cultures have been isolated, they will be analyzed microscopically and identified using molecular methods.

Brock “Biology of Microorganisms”

Cypionka “Grundlagen der Mikrobiologie”

Drews “Mikrobiologisches Praktikum”

DSMZ catalogue (www.dsmz.de)

yer “A field guide to the bacteria”

Reddy “Methods for general and molecular Microbiology”

Steinbüchel “Mikrobiologisches Praktikum”

www.microbiological-garden.net

Aufnahmekapazität:

unbegrenzt

Lehr-/Lernform:

Seminar and laboratory work, twice per week, half a day each 

SE, Ü Isolation and characterisation of microorganisms

Links:

www.microbiological-garden.net