mar600 - Profile Module Methods in Aquatic Microbial Ecology (Vollständige Modulbeschreibung)

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16.09.2021 21:17:48

Modulbezeichnung

Profile Module Methods in Aquatic Microbial Ecology

Modulkürzel

mar600

Kreditpunkte

6.0 KP

Workload

180 h

Einrichtungsverzeichnis

Institut für Chemie und Biologie des Meeres (ICBM)

Verwendbarkeit des Moduls

Master Microbiology (Master) > Mastermodule

Zuständige Personen

Simon, Meinhard (Modulverantwortung)

Brinkhoff, Thorsten Henning (Modulberatung)

Teilnahmevoraussetzungen

For the practical course lecture: Methods in Aquatic Microbial Ecology

Kompetenzziele

Skills to be acquired in this module

The students learn to…

· analyze bacterial substrates at ambient concentrations such as dissolved amino acids and carbohydrates by high performance liquid chromatography (HPLC).

· determine bacterial cell numbers by flow cytometry and epifluorescence microscopy and to analyze these data by image analysis.

· extract bacterial DNA from water and sediment samples.

· amplify bacterial genes by specific primers and PCR.

· assess bacterial communities by culture-independent methods such as denaturing gradient gel electrophoresis and next generation sequencing.

· present and discuss scientific results.

· write a scientific protocol.

The students gain competences in:

· Understanding how to analyze dissolved substrates of heterotrophic aquatic bacterial communities by state of the art approaches.

· How to assess the abundance of aquatic bacterial communities by state of the art approaches.

· Analyzing the composition of bacterial communities by PCR-based culture-independent approaches.

Modulinhalte

The course starts with a lecture introducing basic issues of aquatic microbial ecology with an emphasis on methodological aspects. This lecture is completed before the practical work starts.

During the practical course of a block of two weeks the participants carry out analyses and experiments on:

· determining the concentration of dissolved organic substrates (amino acids, carbohydrates),

· the abundance of bacterial communities in aquatic systems

· the composition of bacterial communities in environmental samples by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA targeted gene fragments.

The main emphasis is on analyses and approaches of bacterial communities in the water column.

Literaturempfehlungen

Lecture notes, available on Stud.IP

Links

Unterrichtssprache

Englisch

Dauer in Semestern

1 Semester

Angebotsrhythmus Modul

jährlich

Aufnahmekapazität Modul

unbegrenzt

Modullevel / module level

Modulart / typ of module

Wahlpflicht / Elective

Lehr-/Lernform / Teaching/Learning method

Block course, 2 weeks; practical course (4 SPPW) and seminar (1 SPPW)

Vorkenntnisse / Previous knowledge

For the practical course lecture: Methods in Aquatic Microbial Ecology

Lehrveranstaltungsform	SWS	Workload Präsenz
Seminar	1	14
Praktikum	4	56
Präsenzzeit Modul insgesamt		70 h

Prüfung	Prüfungszeiten	Prüfungsform
Gesamtmodul	Will be announced during the course.	One assessment of examination: Portfolio (seminar presentation, written protocol) Protocol (100 %), seminar presentation (no mark). Active participation (Active and documented participation in practical courses (labs, exercises, seminars, field trips) and courses. These include e.g. the delivery of exercises, writing a lab report or seminar presentations according to the advice of the course supervisor.)