The course times are not decided yet. gemeinsame Vorbesprechung mit allen Research Module Anbietern in der Woche vor Sommersemester-Beginn (siehe Info-Gruppe des Studiengangs Neuroscience: https://elearning.uni-oldenburg.de/dispatch.php/course/details?sem_id=97e03f9766f14bbfcfea99fc853251af)
The course times are not decided yet. Practical part:
In this module, the influence of agonists of the melanocortin-1 receptor (MC-1R) or the aryl hydrocarbon receptor (AhR) signal pathway on neuronal cells as well as immune cells will be investigated in primary cell cultures. Methodologically, mixed lymphocyte cultures, multicolor flow cytometry, various histological analyses, RNA extraction and quantitative real-time PCR will be used. The practical work will be performed mainly on primary mouse cells.
Theoretical part (seminar):
During the module the pathomechanisms of common CNS diseases (e.g., multiple sclerosis, Alzheimer's disease, Parkinson's disease) will be discussed in detail with a focus on the involvement of the immune system in the development as well as the progression of inflammatory and degenerative CNS diseases. At the same time, new therapeutic concepts will be discussed.
The course times are not decided yet. Practical part:
During infection, bacteria, such as Yersinia enterocolitica, can actively translocate anti-inflammatory proteins into the host cell cytoplasm to suppress the host immune response. These proteins can be produced recombinantly and are to be formulated as drugs for therapy. For this purpose, the mouse model of imiquimod-induced psoriasis-like skin inflammation was used, and the animals were treated with the bacterial compounds. After the efficacy has been proven based on the reduction in disease severity, the mechanism of action shall be analyzed in detail. For this purpose, the induction of apoptosis, the inflammasome activation, cytokine production and NF-B activation or the effect of the proteins on the integrity of the epithelial barrier will be analyzed within this research module. Methods such as multicolor immunofluorescence, multiplex assays for cytokine quantification and characterization of signaling cascades, RNA sequencing or kinome analysis will be used.
Theoretical part (seminar):
During the research module, the pathomechanisms of chronic inflammatory skin diseases will be discussed, with a specific focus on the involvement of the immune system in the development of psoriasis vulgaris. At the same time, various possibilities how bacterial pathogens evade host immune defenses will be presented. These possibilities include the production of cell-penetrating peptides (CPEs) with anti-inflammatory properties, which can be developed as agents for therapy (drugs-from-bugs concept).
The course times are not decided yet. Practical part:
In this module, the efficacy of environmental mimetics in a model system of multiple sclerosis will be investigated. Methodologically, a mouse model system of multiple sclerosis mixed, multicolor flow cytometry, various histological analyses, RNA extraction and quantitative real-time PCR will be used. The practical work will be performed mainly on primary mouse cells.
Theoretical part (seminar):
During the module the pathomechanisms of common CNS diseases (e.g., multiple sclerosis, Alzheimer's disease, Parkinson's disease) will be discussed in detail with a focus on the involvement of the immune system in the development as well as the progression of inflammatory and degenerative CNS diseases. At the same time, the relevance of environmental factors on these diseases will be discussed as well as the possibilities of environmental mimetic as therapeutic concepts.
The course times are not decided yet. gemeinsame Vorbesprechung mit allen Research Module Anbietern in der Woche vor Sommersemester-Beginn (siehe Info-Gruppe des Studiengangs Neuroscience: https://elearning.uni-oldenburg.de/dispatch.php/course/details?sem_id=97e03f9766f14bbfcfea99fc853251af)
The course times are not decided yet. Practical part:
In this module, the influence of agonists of the melanocortin-1 receptor (MC-1R) or the aryl hydrocarbon receptor (AhR) signal pathway on neuronal cells as well as immune cells will be investigated in primary cell cultures. Methodologically, mixed lymphocyte cultures, multicolor flow cytometry, various histological analyses, RNA extraction and quantitative real-time PCR will be used. The practical work will be performed mainly on primary mouse cells.
Theoretical part (seminar):
During the module the pathomechanisms of common CNS diseases (e.g., multiple sclerosis, Alzheimer's disease, Parkinson's disease) will be discussed in detail with a focus on the involvement of the immune system in the development as well as the progression of inflammatory and degenerative CNS diseases. At the same time, new therapeutic concepts will be discussed.
The course times are not decided yet. Practical part:
During infection, bacteria, such as Yersinia enterocolitica, can actively translocate anti-inflammatory proteins into the host cell cytoplasm to suppress the host immune response. These proteins can be produced recombinantly and are to be formulated as drugs for therapy. For this purpose, the mouse model of imiquimod-induced psoriasis-like skin inflammation was used, and the animals were treated with the bacterial compounds. After the efficacy has been proven based on the reduction in disease severity, the mechanism of action shall be analyzed in detail. For this purpose, the induction of apoptosis, the inflammasome activation, cytokine production and NF-B activation or the effect of the proteins on the integrity of the epithelial barrier will be analyzed within this research module. Methods such as multicolor immunofluorescence, multiplex assays for cytokine quantification and characterization of signaling cascades, RNA sequencing or kinome analysis will be used.
Theoretical part (seminar):
During the research module, the pathomechanisms of chronic inflammatory skin diseases will be discussed, with a specific focus on the involvement of the immune system in the development of psoriasis vulgaris. At the same time, various possibilities how bacterial pathogens evade host immune defenses will be presented. These possibilities include the production of cell-penetrating peptides (CPEs) with anti-inflammatory properties, which can be developed as agents for therapy (drugs-from-bugs concept).
The course times are not decided yet. Practical part:
In this module, the efficacy of environmental mimetics in a model system of multiple sclerosis will be investigated. Methodologically, a mouse model system of multiple sclerosis mixed, multicolor flow cytometry, various histological analyses, RNA extraction and quantitative real-time PCR will be used. The practical work will be performed mainly on primary mouse cells.
Theoretical part (seminar):
During the module the pathomechanisms of common CNS diseases (e.g., multiple sclerosis, Alzheimer's disease, Parkinson's disease) will be discussed in detail with a focus on the involvement of the immune system in the development as well as the progression of inflammatory and degenerative CNS diseases. At the same time, the relevance of environmental factors on these diseases will be discussed as well as the possibilities of environmental mimetic as therapeutic concepts.
as defined in the admission and examination regulations
Module examination
graded: project report
ungraded: participation in seminar and 30 min. presentation
Skills to be acquired in this module
Competencies:
++ deepened biological and / or clinical expertise
++ deepened knowledge of biological working methods and / or clinical diagnostics
++ data analysis skills
+ interdisciplinary thinking
++ critical and analytical thinking
++ independent searching and knowledge of scientific literature
++ ability to perform independent biological research
++ data presentation and discussion (written and spoken)
+ team work
+ ethics and professional behaviour
+ project and time management
